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rabbit polyclonal antibody to top2a proteintech 20233 1 ap at 1 400 dilution  (Proteintech)


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    Structured Review

    Proteintech rabbit polyclonal antibody to top2a proteintech 20233 1 ap at 1 400 dilution
    Spatial transcriptomics-based in situ hybridization–like expression mapping of the <t>TOP2A-LGALS1-immune</t> microenvironments axis and validation by immunohistochemistry. ( A ) To simulate an in situ hybridization–like pattern, spatial transcriptomic data for TOP2A, LGALS1, CD69, and CD4 were mapped onto the tissue section. TOP2A and LGALS1 expression was markedly upregulated in the tumor region compared with the non-tumorous area located in the lower right of the section. In contrast, no corresponding increase in the expression of CD69 or CD4, which are presumed to be negatively regulated by LGALS1, was observed. Bars = 1 mm. ( B ) Immunohistochemical staining using specific antibodies against TOP2A, LGALS1, CD69, and CD4 demonstrated expression patterns parallel to those observed in panel ( A ), confirming that the mRNA expression profiles identified by spatial transcriptomics are similarly reflected at the protein level. Microvessels are indicated by arrows. Bars = 75 μm.
    Rabbit Polyclonal Antibody To Top2a Proteintech 20233 1 Ap At 1 400 Dilution, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+to+top2a+proteintech+20233+1+ap+at+1+400+dilution/TOP2A-Specific+Antibody/pmc12971178-78-7-12
    Average 94 stars, based on 28 article reviews
    rabbit polyclonal antibody to top2a proteintech 20233 1 ap at 1 400 dilution - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Spatial Transcriptomics Meets Histochemistry: Insights from Glioblastoma as a Model System"

    Article Title: Spatial Transcriptomics Meets Histochemistry: Insights from Glioblastoma as a Model System

    Journal: Acta Histochemica et Cytochemica

    doi: 10.1267/ahc.25-00065

    Spatial transcriptomics-based in situ hybridization–like expression mapping of the TOP2A-LGALS1-immune microenvironments axis and validation by immunohistochemistry. ( A ) To simulate an in situ hybridization–like pattern, spatial transcriptomic data for TOP2A, LGALS1, CD69, and CD4 were mapped onto the tissue section. TOP2A and LGALS1 expression was markedly upregulated in the tumor region compared with the non-tumorous area located in the lower right of the section. In contrast, no corresponding increase in the expression of CD69 or CD4, which are presumed to be negatively regulated by LGALS1, was observed. Bars = 1 mm. ( B ) Immunohistochemical staining using specific antibodies against TOP2A, LGALS1, CD69, and CD4 demonstrated expression patterns parallel to those observed in panel ( A ), confirming that the mRNA expression profiles identified by spatial transcriptomics are similarly reflected at the protein level. Microvessels are indicated by arrows. Bars = 75 μm.
    Figure Legend Snippet: Spatial transcriptomics-based in situ hybridization–like expression mapping of the TOP2A-LGALS1-immune microenvironments axis and validation by immunohistochemistry. ( A ) To simulate an in situ hybridization–like pattern, spatial transcriptomic data for TOP2A, LGALS1, CD69, and CD4 were mapped onto the tissue section. TOP2A and LGALS1 expression was markedly upregulated in the tumor region compared with the non-tumorous area located in the lower right of the section. In contrast, no corresponding increase in the expression of CD69 or CD4, which are presumed to be negatively regulated by LGALS1, was observed. Bars = 1 mm. ( B ) Immunohistochemical staining using specific antibodies against TOP2A, LGALS1, CD69, and CD4 demonstrated expression patterns parallel to those observed in panel ( A ), confirming that the mRNA expression profiles identified by spatial transcriptomics are similarly reflected at the protein level. Microvessels are indicated by arrows. Bars = 75 μm.

    Techniques Used: Spatial Transcriptomics, In Situ Hybridization, Expressing, Biomarker Discovery, Immunohistochemistry, Immunohistochemical staining, Staining

    Related Articles

    Immunohistochemical staining:

    Article Title: Spatial Transcriptomics Meets Histochemistry: Insights from Glioblastoma as a Model System
    Article Snippet: .. Importantly, subsequent immunohistochemical analysis by use of (rabbit polyclonal antibody to TOP2A (Proteintech, 20233-1-AP, at 1:400 dilution) and to Galectin-1 (Proteintech, 11858-1-AP, at 1:1000 dilution) provided additional spatial resolution at the protein level, demonstrating that both TOP2A and LGALS1 showed particularly enhanced expression in tumor cells located in close proximity to microvessels ( B). ..

    Expressing:

    Article Title: Spatial Transcriptomics Meets Histochemistry: Insights from Glioblastoma as a Model System
    Article Snippet: .. Importantly, subsequent immunohistochemical analysis by use of (rabbit polyclonal antibody to TOP2A (Proteintech, 20233-1-AP, at 1:400 dilution) and to Galectin-1 (Proteintech, 11858-1-AP, at 1:1000 dilution) provided additional spatial resolution at the protein level, demonstrating that both TOP2A and LGALS1 showed particularly enhanced expression in tumor cells located in close proximity to microvessels ( B). ..



    Similar Products

    rabbit polyclonal antibody to top2a proteintech 20233 1 ap at 1 400 dilution  (Proteintech)
    94
    Proteintech rabbit polyclonal antibody to top2a proteintech 20233 1 ap at 1 400 dilution
    Spatial transcriptomics-based in situ hybridization–like expression mapping of the <t>TOP2A-LGALS1-immune</t> microenvironments axis and validation by immunohistochemistry. ( A ) To simulate an in situ hybridization–like pattern, spatial transcriptomic data for TOP2A, LGALS1, CD69, and CD4 were mapped onto the tissue section. TOP2A and LGALS1 expression was markedly upregulated in the tumor region compared with the non-tumorous area located in the lower right of the section. In contrast, no corresponding increase in the expression of CD69 or CD4, which are presumed to be negatively regulated by LGALS1, was observed. Bars = 1 mm. ( B ) Immunohistochemical staining using specific antibodies against TOP2A, LGALS1, CD69, and CD4 demonstrated expression patterns parallel to those observed in panel ( A ), confirming that the mRNA expression profiles identified by spatial transcriptomics are similarly reflected at the protein level. Microvessels are indicated by arrows. Bars = 75 μm.
    Rabbit Polyclonal Antibody To Top2a Proteintech 20233 1 Ap At 1 400 Dilution, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+to+top2a+proteintech+20233+1+ap+at+1+400+dilution/TOP2A-Specific+Antibody/pmc12971178-78-7-12
    Average 94 stars, based on 1 article reviews
    rabbit polyclonal antibody to top2a proteintech 20233 1 ap at 1 400 dilution - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Spatial transcriptomics-based in situ hybridization–like expression mapping of the TOP2A-LGALS1-immune microenvironments axis and validation by immunohistochemistry. ( A ) To simulate an in situ hybridization–like pattern, spatial transcriptomic data for TOP2A, LGALS1, CD69, and CD4 were mapped onto the tissue section. TOP2A and LGALS1 expression was markedly upregulated in the tumor region compared with the non-tumorous area located in the lower right of the section. In contrast, no corresponding increase in the expression of CD69 or CD4, which are presumed to be negatively regulated by LGALS1, was observed. Bars = 1 mm. ( B ) Immunohistochemical staining using specific antibodies against TOP2A, LGALS1, CD69, and CD4 demonstrated expression patterns parallel to those observed in panel ( A ), confirming that the mRNA expression profiles identified by spatial transcriptomics are similarly reflected at the protein level. Microvessels are indicated by arrows. Bars = 75 μm.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Spatial Transcriptomics Meets Histochemistry: Insights from Glioblastoma as a Model System

    doi: 10.1267/ahc.25-00065

    Figure Lengend Snippet: Spatial transcriptomics-based in situ hybridization–like expression mapping of the TOP2A-LGALS1-immune microenvironments axis and validation by immunohistochemistry. ( A ) To simulate an in situ hybridization–like pattern, spatial transcriptomic data for TOP2A, LGALS1, CD69, and CD4 were mapped onto the tissue section. TOP2A and LGALS1 expression was markedly upregulated in the tumor region compared with the non-tumorous area located in the lower right of the section. In contrast, no corresponding increase in the expression of CD69 or CD4, which are presumed to be negatively regulated by LGALS1, was observed. Bars = 1 mm. ( B ) Immunohistochemical staining using specific antibodies against TOP2A, LGALS1, CD69, and CD4 demonstrated expression patterns parallel to those observed in panel ( A ), confirming that the mRNA expression profiles identified by spatial transcriptomics are similarly reflected at the protein level. Microvessels are indicated by arrows. Bars = 75 μm.

    Article Snippet: Importantly, subsequent immunohistochemical analysis by use of (rabbit polyclonal antibody to TOP2A (Proteintech, 20233-1-AP, at 1:400 dilution) and to Galectin-1 (Proteintech, 11858-1-AP, at 1:1000 dilution) provided additional spatial resolution at the protein level, demonstrating that both TOP2A and LGALS1 showed particularly enhanced expression in tumor cells located in close proximity to microvessels ( B).

    Techniques: Spatial Transcriptomics, In Situ Hybridization, Expressing, Biomarker Discovery, Immunohistochemistry, Immunohistochemical staining, Staining